corolase pp  (AB Enzymes)

Journal: Foods

Article Title: Exploring the Potential of Atlantic Mesopelagic Species Processed on Board Commercial Fishing Vessels as a Source of Dietary Lipids

doi: 10.3390/foods13071094

Figure Lengend Snippet: Mass balance of the fractions obtained after processing with thermal treatment and enzymatic hydrolysis with commercial and endogenous enzymes. Values are shown as a percentage of the wet weight of raw material (g/100 g). T, E, PB, P, and C refer to thermal separation, endogenous enzymes, papain-bromelain, Protamex, and Corolase, respectively. The data are presented as mean values, and the minimum and maximum values are included in brackets. The variance among the different treatments ( p < 0.05) is described with the symbols “ <” and “> ” that indicate if the value on the left is significantly higher or smaller than the value on the right, respectively.

Article Snippet: Corolase PP (AB Enzymes GmbH, Darmstadt, Germany), Protamex (Novozymes A/S, Bagsvaerd, Denmark), and papain and bromelain (tested as a 50:50 mixture; Enzybel International, Waterloo, Belgium) were purchased from the respective manufacturers.

Techniques: Raw Material, Emulsion

Journal: Molecules

Article Title: Streptococcus thermophilus : A Source of Postbiotics Displaying Anti-Inflammatory Effects in THP 1 Macrophages

doi: 10.3390/molecules29071552

Figure Lengend Snippet: Proteins identified by LC-MS/MS after Corolase PP hydrolysis of 3–10 kDa intracellular protein (IP) fractions of S. thermophilus LMD-9 (IP-9) and CNRZ-21N (IP-21).

Article Snippet: The hydrolysates obtained after Corolase PP hydrolysis of these intracellular proteins could be considered as postbiotics with potential anti-inflammatory effects for human health, which could make them promising bioactive ingredients for the development of functional foods aimed at preventing pathologies with an inflammatory component.

Techniques:

Journal: Molecules

Article Title: Streptococcus thermophilus : A Source of Postbiotics Displaying Anti-Inflammatory Effects in THP 1 Macrophages

doi: 10.3390/molecules29071552

Figure Lengend Snippet: Effects of IPH-9 and IPH-21, obtained after hydrolysis by Corolase PP of IP-9 and IP-21 fractions from the S. thermophilus LMD-9 and CNRZ-21N strains, respectively, on the secretion of ( A ) IL-1β, ( B ) IL-8 and ( C ) TNF-α cytokines in LPS-inflamed THP-1 macrophages. Cells were co-incubated with LPS (50 ng/mL) and with or without IPH-9 or IPH-21 (0.5 or 1 mg/mL) for 3 h. The negative control (LPS−) corresponds to untreated cells. Dexamethasone (DEX) at 25 µM was used as a positive control (gray bars without pattern). IL-8 (%), TNF-α (%) and IL-1β (%) are the percentages of IL-8, TNF-α or IL-1β, respectively, secreted by cells compared to their secretions by cells treated only with LPS (100% control; gray hatch filled bars). Results are expressed as means ± SEM. * p < 0.05, **** p < 0.0001.

Article Snippet: The hydrolysates obtained after Corolase PP hydrolysis of these intracellular proteins could be considered as postbiotics with potential anti-inflammatory effects for human health, which could make them promising bioactive ingredients for the development of functional foods aimed at preventing pathologies with an inflammatory component.

Techniques: Incubation, Negative Control, Positive Control, Control

Journal: Molecules

Article Title: Streptococcus thermophilus : A Source of Postbiotics Displaying Anti-Inflammatory Effects in THP 1 Macrophages

doi: 10.3390/molecules29071552

Figure Lengend Snippet: Effects of IPH-9 and IPH-21, obtained after Corolase PP hydrolysis of IP-9 and IP-21 fractions from the S. thermophilus LMD-9 and CNRZ-21N strain, respectively, on COX-2 and pro-IL-1β protein expression in LPS-inflamed THP-1 macrophages. ( A – C ) THP-1 macrophages were co-incubated with LPS (50 ng/mL) alone (gray hatch filled bars) or with LPS and IPH-9 or IPH-21 at (0.5 or 1.0 mg/mL) for 3 h. Dexamethasone (DEX) at 25 µM was used as a positive control (gray bars without pattern). Fifteen µg of each protein sample was loaded onto a 10% polyacrylamide gel containing SDS. The results were calculated as a relative intensity to the β-actin. Results are expressed as means ± SEM. * p < 0.05, *** p < 0.001, **** p < 0.0001.

Article Snippet: The hydrolysates obtained after Corolase PP hydrolysis of these intracellular proteins could be considered as postbiotics with potential anti-inflammatory effects for human health, which could make them promising bioactive ingredients for the development of functional foods aimed at preventing pathologies with an inflammatory component.

Techniques: Expressing, Incubation, Positive Control

Journal: Molecules

Article Title: Streptococcus thermophilus : A Source of Postbiotics Displaying Anti-Inflammatory Effects in THP 1 Macrophages

doi: 10.3390/molecules29071552

Figure Lengend Snippet: Proteins identified by LC-MS/MS after Corolase PP hydrolysis of 3–10 kDa intracellular protein (IP) fractions of S. thermophilus LMD-9 (IP-9) and CNRZ-21N (IP-21).

Article Snippet: IP-9 and IP-21 fractions were solubilized in 10 mM PBS (137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 2 mM KH 2 PO 4 ), pH 7.4 and were hydrolysed with the pancreatic-enzyme complex, Corolase PP (AB Enzymes GmbH, Darmstadt, Germany).

Techniques:

Journal: Molecules

Article Title: Streptococcus thermophilus : A Source of Postbiotics Displaying Anti-Inflammatory Effects in THP 1 Macrophages

doi: 10.3390/molecules29071552

Figure Lengend Snippet: Effects of IPH-9 and IPH-21, obtained after hydrolysis by Corolase PP of IP-9 and IP-21 fractions from the S. thermophilus LMD-9 and CNRZ-21N strains, respectively, on the secretion of ( A ) IL-1β, ( B ) IL-8 and ( C ) TNF-α cytokines in LPS-inflamed THP-1 macrophages. Cells were co-incubated with LPS (50 ng/mL) and with or without IPH-9 or IPH-21 (0.5 or 1 mg/mL) for 3 h. The negative control (LPS−) corresponds to untreated cells. Dexamethasone (DEX) at 25 µM was used as a positive control (gray bars without pattern). IL-8 (%), TNF-α (%) and IL-1β (%) are the percentages of IL-8, TNF-α or IL-1β, respectively, secreted by cells compared to their secretions by cells treated only with LPS (100% control; gray hatch filled bars). Results are expressed as means ± SEM. * p < 0.05, **** p < 0.0001.

Article Snippet: IP-9 and IP-21 fractions were solubilized in 10 mM PBS (137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 2 mM KH 2 PO 4 ), pH 7.4 and were hydrolysed with the pancreatic-enzyme complex, Corolase PP (AB Enzymes GmbH, Darmstadt, Germany).

Techniques: Incubation, Negative Control, Positive Control, Control

Journal: Molecules

Article Title: Streptococcus thermophilus : A Source of Postbiotics Displaying Anti-Inflammatory Effects in THP 1 Macrophages

doi: 10.3390/molecules29071552

Figure Lengend Snippet: Effects of IPH-9 and IPH-21, obtained after Corolase PP hydrolysis of IP-9 and IP-21 fractions from the S. thermophilus LMD-9 and CNRZ-21N strain, respectively, on COX-2 and pro-IL-1β protein expression in LPS-inflamed THP-1 macrophages. ( A – C ) THP-1 macrophages were co-incubated with LPS (50 ng/mL) alone (gray hatch filled bars) or with LPS and IPH-9 or IPH-21 at (0.5 or 1.0 mg/mL) for 3 h. Dexamethasone (DEX) at 25 µM was used as a positive control (gray bars without pattern). Fifteen µg of each protein sample was loaded onto a 10% polyacrylamide gel containing SDS. The results were calculated as a relative intensity to the β-actin. Results are expressed as means ± SEM. * p < 0.05, *** p < 0.001, **** p < 0.0001.

Article Snippet: IP-9 and IP-21 fractions were solubilized in 10 mM PBS (137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 2 mM KH 2 PO 4 ), pH 7.4 and were hydrolysed with the pancreatic-enzyme complex, Corolase PP (AB Enzymes GmbH, Darmstadt, Germany).

Techniques: Expressing, Incubation, Positive Control